Identification of a Morphogenic Intermediate of the Bacteriophage Mu Baseplate

Autor:	Keiko Yoshida, Shigeki Takeda, Yuzuki Shimamori, Yuko Kanazawa, Nao Tsukamoto
Rok vydání:	2014
Předmět:	biology Chemistry Dimer Mutant Myoviridae General Medicine biology.organism_classification Bioinformatics Genome Bacteriophage chemistry.chemical_compound Monomer Biochemistry Bacteriophage Mu Gene
Zdroj:	Advances in Microbiology. :1155-1163
ISSN:	2165-3410 2165-3402
DOI:	10.4236/aim.2014.416125
Popis:	Bacteriophage morphogenesis is a model system for investigating sequential molecular assembly. The Mu phage is one of the most classical Myoviridae. Although it is well known as a mobile genetic element, the details of its morphogenesis remain unclear. Analysis of conditional lethal mutants and genome analysis of the Mu phage have suggested that genes 42, 43, 44, 45, 46, 47, and 48 are essential for its baseplate assembly. Since we have already reported X-ray structures of the products of genes 44 (gp44) and 45 (gp45), we here tried to purify the remaining Mu phage baseplate subunits, gp42, gp43, gp46, gp47, and gp48, to investigate the baseplate assembly process. In the case of gp42 expression, the transformed E. coli cells showed growth inhibition after induction and no gp42 fractions were observed. However, gp43, gp46, gp47, and gp48 were successfully expressed and purified, although gp48 could not be applied to further analysis, because the amount of soluble fraction was very low. Based on analytical ultracentrifugation, we concluded that gp43 formed a monomer, gp46 was a monomer, and gp47 occurred as both a monomer and dimer in solution. Moreover, we found that gp43 and gp45 formed an intermediate complex in the baseplate assembly process.
Databáze:	OpenAIRE
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