Low dose-rate irradiation with [3H]-labelled valine to selectively target hypoxic cells in a human colorectal cancer xenograft model

Autor: Siri Fenne, Joe Alexander Sandvik, Heidi Lyng, Kjersti Flatmark, Nina Jeppesen Edin, Lars Tore Gyland Mikalsen, Stine Gyland Mikalsen, Erik O. Pettersen, Eva Katrine Aarnes
Rok vydání: 2018
Předmět:
Zdroj: Acta Oncologica. 57:1216-1224
ISSN: 1651-226X
0284-186X
DOI: 10.1080/0284186x.2018.1457223
Popis: Background: Earlier in vitro studies show that irradiation with an ultra-low dose-rate of 15 mGy/h delivered with [3H]-valine leads to loss of clonogenicity in hypoxic T-47D cells. Here, the aim was to determine if [3H]-valine could be used to deliver low dose-rate irradiation in a colorectal cancer model.Methods: Clonogenicity was measured in cultured cancer cell line HT29 irradiated with 15 mGy/h combined with intermittent hypoxia. Mice with HT29 xenografts were irradiated by repeated injections of [3H]-valine intravenously. The activity in the tumor tissue was measured by scintillation counting and tumor growth, hypoxic fraction and tritium distribution within tumors were assessed by pimonidazole staining and autoradiography.Results: Ultra-low dose-rate irradiation decreased clonogenicity in hypoxic colorectal cancer cells. In vivo, the tumor growth, hypoxic fraction and weight of the mice were similar between the treated and untreated group. Autoradiography showed no [3H]-valine uptake in hypo...
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje