Close Spectroscopic Look at Dye-Stained Polymer Microbeads
| Autor: | Katrin Hoffmann, Uwe Schedler, Ute Resch-Genger, Daniel Kage, Linn Fischer, Thomas Thiele |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Fluorophore Materials science 02 engineering and technology Polymer Bead 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Rhodamine 6G chemistry.chemical_compound General Energy chemistry visual_art Biophysics Fluorescence microscope visual_art.visual_art_medium Particle Physical and Theoretical Chemistry 0210 nano-technology Fluorescence anisotropy |
| Zdroj: | The Journal of Physical Chemistry C. 122:12782-12791 |
| ISSN: | 1932-7455 1932-7447 |
| DOI: | 10.1021/acs.jpcc.8b02546 |
| Popis: | Dye-stained micrometer-sized polymer beads are important tools in the life sciences with applications in biomedical, biochemical, and clinical research. Here, bead-based assays are increasingly used, for example, in DNA sequencing and the detection of autoimmune diseases or pathogenic microorganisms. Moreover, stained beads are employed as calibration tools for fluorescence microscopy and flow cytometry methods with increasing complexity. To address the requirements concerning the relevant fluorescence features, the spectroscopic properties of representative polymer beads with diameters ranging from about 1 to 10 μm stained with varying concentrations of rhodamine 6G were systematically assessed. The observed dependence of the spectral properties, fluorescence decay kinetics, and fluorescence quantum yields on bead size and dye loading concentration is attributed to different fluorescence characteristics of fluorophores located in the particle core and near-surface dye molecules. Supported by the fluorescence anisotropy measurements, the origin of the observed alteration of fluorescence features is ascribed to a combination of excitation energy transfer and polarity-related effects that are especially pronounced at the interface of the bead and the surrounding medium. The results of our studies underline the need to carefully control and optimize all Parameters that can affect the fluorescence properties of the dye-stained beads. |
| Databáze: | OpenAIRE |
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