Milk protein profiles in response toStreptococcus agalactiaesubclinical mastitis in dairy cows
| Autor: | Pongphol Pongthaisong, Sittiruk Roytrakul, Chaiyapas Thamrongyoswittayakul, Suporn Katawatin |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Gel electrophoresis Milk protein Mammary gland 0402 animal and dairy science food and beverages 04 agricultural and veterinary sciences General Medicine Biology Proteomics medicine.disease_cause 040201 dairy & animal science Microbiology 03 medical and health sciences fluids and secretions 030104 developmental biology medicine.anatomical_structure Streptococcus agalactiae Immunology medicine Biomarker (medicine) General Agricultural and Biological Sciences Somatic cell count Subclinical infection |
| Zdroj: | Animal Science Journal. 87:92-98 |
| ISSN: | 1344-3941 |
| DOI: | 10.1111/asj.12391 |
| Popis: | The objective of this study was to investigate the milk protein profiles of normal milk and those of milk during the course of subclinical mastitis, caused by natural Streptococcus agalactiae infection. Two-dimensional gel electrophoresis and liquid chromatography mass spectrometry were used to assess protein profiles and to identify the proteins. The results showed that S. agalactiae subclinical mastitis altered the protein profiles of milk. Following Mascot database matching, 11 and 12 protein types were identified in the milk collected from healthy and S. agalactiae subclinical mastitic udders, respectively. The distinct presence of the antibacterial protein cathelicidin-1 was detected in infected milk samples, which in turn was highly correlated to the severity of subclinical mastitis as represented by the milk somatic cell count (r = 0.616), but not the bacterial count. The protein profile of milk reveals changes in the host response to S. agalactiae intramammary infection; cathelicidin-1 could therefore serve as a biomarker for the detection of subclinical mastitis in dairy cows. |
| Databáze: | OpenAIRE |
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