Validation of a method for the determination of 120 pesticide residues in apples and cucumbers by LC-MS/MS
| Autor: | Gouda Ramadan, Ali Saad Mohammed, Muna Al Jabir, Najat A. Alabdulmalik |
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| Rok vydání: | 2016 |
| Předmět: |
Detection limit
Pesticide residue business.industry 010401 analytical chemistry Pharmaceutical Science 04 agricultural and veterinary sciences Pesticide Quechers Food safety 040401 food science 01 natural sciences 0104 chemical sciences Analytical Chemistry Toxicology 0404 agricultural biotechnology Food products Lc ms ms Environmental Chemistry Environmental science business Spectroscopy |
| Zdroj: | Drug Testing and Analysis. 8:498-510 |
| ISSN: | 1942-7603 |
| DOI: | 10.1002/dta.2008 |
| Popis: | Most countries have clearly defined regulations governing the use of pesticides in agricultural activity. The application of pesticides in agriculture usually leads to a residual amount of these pesticides on food products such as fruit and vegetables. The presence of pesticide residues on these foods destined for human consumption may pose food safety risks to consumers. To protect consumers, national authorities have established maximum limits for pesticide residues in foods. These limits can only be enforced if there are methods available to detect and monitor their concentrations in the applicable food products. To support the enforcement of this legislation, we have developed a multi-residue liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the analysis of 120 pesticide residues in apples and cucumbers which has been validated and implemented in the routine monitoring and surveillance programme for these pesticides. In this method, apple and cucumber samples are extracted using the QuEChERS method (quick, easy, cheap, effective, rugged, and safe) and the extracts were analyzed directly by LC-MS/MS. The mean recoveries at three different concentrations of 0.01 µg/g , 0.05 µg/g, and 0.1 µg/g over the analytical range varied between 70 and 120%. The repeatability of the method expressed as %RSD was less than 20%. The limit of detection (LOD) of the method ranged between 0.0014 and 0.0110 µg/g for apples and between 0.0012 and 0.0075 µg/g for cucumbers. The limit of quantification (LOQ) of the method was 0.01 µg/g for apples and cucumbers. The method has been used for the analysis of over 600 apple and 550 cucumber samples over the past two years. Copyright © 2016 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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