NADH-cytochrome c reductase activity in cultured human lymphocytes

Autor:	Richard A. Kouri, Richard L. Imblum, Russell A. Prough
Rok vydání:	1976
Předmět:	7-Dehydrocholesterol reductase Cytochrome b Lymphocyte Biophysics Cytochrome P450 reductase Antimycin A Reductase Biology Biochemistry Molecular biology chemistry.chemical_compound medicine.anatomical_structure chemistry Cytochrome b5 medicine Microsome Molecular Biology
Zdroj:	Archives of Biochemistry and Biophysics. 176:119-126
ISSN:	0003-9861
DOI:	10.1016/0003-9861(76)90147-8
Popis:	A NADH-cytochrome c reductase activity was increased upon mitogen stimulation of human lymphocytes. The activity was not inhibited by antimycin A or rotenone but was specifically inhibited by antibodies elicited against rat liver NADH-cytochrome b 5 reductase or cytochrome b 5 . The activity was linear with cellular homogenates up to 5.2 × 10 6 cells/ml and had abroad pH optimum of 7.7. The presence of 3-methylcholanthrene in mitogen stimulation media had no effect on the NADH-cytochrome c reductase activity but differentially induced the benzo(a)pyrene hydroxylase (AHH) activity. The reductase activity was present in nonstimulated cells and appears not to be significantly increased in activity per cell upon mitogen-stimulation of the peripheral lymphocyte.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8c728c934d89fcd0ef5a2e62adf25c0f https://doi.org/10.1016/0003-9861(76)90147-8 Zobrazit plný text záznamu