Characterization of M cell development during indomethacin-induced ileitis in rats

Autor:	Christoph Cichon, Torsten Kucharzik, Norbert Lügering, Reichelt R, M. A. Schmidt, Andreas Lügering, Wolfram Domschke, R. Stoll, M. Linnepe, K. Rautenberg
Rok vydání:	2000
Předmět:	Pathology medicine.medical_specialty Lamina propria Hepatology Gastroenterology Inflammation Biology medicine.disease Epithelium medicine.anatomical_structure Immune system Transcytosis Apoptosis medicine Pharmacology (medical) Ileitis medicine.symptom Microfold cell
Zdroj:	Alimentary Pharmacology & Therapeutics. 14:247-256
ISSN:	0269-2813
Popis:	Background : M cells play an important role in the intestinal immune system as they have a high capacity for transcytosis of a wide range of microorganisms and macromolecules. However, little is known about the role of M cells during intestinal inflammation. Aim : We studied M cell development during indomethacin-induced intestinal inflammation in rats. Methods : Ileitis in rats was induced by two subcutaneous injections with indomethacin (7.5 mg/kg) given 24 h apart. Rats were sacrificed after 14 days and tissue was analysed by fluorescence microscopy and electron microscopy. M cells could be visualized by using the FITC-labelled mAb anti-cytokeratin (CK)-8 (clone 4.1.18), which was recently identified as specific M cell marker in rats. The number of cytokeratin-8 positive M cells was related to the surface of the follicle associated epithelium. For morphological studies, we used both transmission electron microscopy (T.E.M.) and scanning electron microscopy (S.E.M.). Results : In non-inflamed ileum M cells were scarce. Only 4% of the follicle associated epithelium were M cells, whereas an increase of M cells up to 11% was found in inflamed follicle associated epithelium (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8c674ca5758ab758b7eb75e06318f8f6 https://doi.org/10.1046/j.1365-2036.2000.00688.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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