Use of nystatin for selection of tributyrin non-utilising mutants in Yarrowia lipolytica
| Autor: | E.W. Lim, P.C. Nai, Claude Gaillardin, B. H. Nga, K. Chan, P. Fournier, Henri Heslot, Y.N. Chan |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
biology
Strain (chemistry) Tributyrin Mutant Bioengineering Yarrowia General Medicine biology.organism_classification Applied Microbiology and Biotechnology chemistry.chemical_compound Nystatin Biochemistry chemistry medicine Extracellular biology.protein Lipase Selection (genetic algorithm) Biotechnology medicine.drug |
| Zdroj: | Journal of Biotechnology. 7:83-86 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/0168-1656(88)90037-5 |
| Popis: | UV mutagenesis of the strain 1529 A his 1 of Yarrowia lipolytica to give a 20% survival provided genetic variants for selection of tributyrin non-utilising mutants. Nystatin selection procedure gave an enhanced efficiency of recovery of tributyrin non-utilising mutants. The mutants isolated reverted at frequencies which ranged from 10 −7 –10 −9 per cell plated. Eight of the mutants were assayed for extracellular lipase activity and gave low lipase activity when compared with the parent strain 1529 A his 1 . |
| Databáze: | OpenAIRE |
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