Effect of Elevated Atmospheric Pressure on Penicillin Binding by Staphylococcus aureus and Streptococcus pyogenes
| Autor: | N. A. Schlamm, O. P. Daily |
|---|---|
| Rok vydání: | 1973 |
| Předmět: | |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 3:147-151 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.3.2.147 |
| Popis: | A gas pressure of 68 atm, elicited by helium-oxygen gas mixtures, reduced the susceptibility to penicillin of Staphylococcus aureus but not of Streptococcus pyogenes . The elevated pressure also caused a reduction in the binding of 14 C-penicillin to S. aureus , but not to S. pyogenes . When these studies were extended to glycine incorporation, it was shown that, even without penicillin, pressurization reduced glycine incorporation into the cell wall of S. aureus . Incorporation into other cellular components was not altered by pressurization. Cells grown in a pressurized environment were slightly more susceptible than those grown at 1 atm to rapid change in osmotic pressure. In the presence of penicillin, glycine incorporation into the cell wall was reduced to the same low level at 68 atm and at 1 atm. These results suggest that pressurization renders S. aureus less susceptible to penicillin because it reduces the enzymatic activity of the binding component on the cell, a penicillin-sensitive transpeptidase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|