Determination of pesticides using a low-temperature co-fired ceramic microfluidic platform
| Autor: | Francisco Céspedes, R. Montes, Mireia Baeza |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Detection limit
Chromatography Materials science General Chemical Engineering 010401 analytical chemistry Microfluidics Substrate (chemistry) 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Amperometry 0104 chemical sciences visual_art Acetylthiocholine visual_art.visual_art_medium Ceramic Co-fired ceramic 0210 nano-technology Instrumentation Biosensor General Environmental Science |
| Zdroj: | Instrumentation Science & Technology. 46:76-92 |
| ISSN: | 1525-6030 1073-9149 |
| DOI: | 10.1080/10739149.2017.1328692 |
| Popis: | The aim of this work is the integration of an improved graphite–epoxy composite electrode modified with the acetylcholinesterase enzyme as a detector into a green tape ceramic microfluidic device for the online amperometric determination of pesticides. First, the operational conditions of the microfluidic system were optimized for the enzymatic substrate determination of acetylthiocholine. Good results were achieved for acetylthiocholine determination, obtaining a low detection limit and optimal sensitivity. The analytical performance of the microanalyzer was evaluated with organophosphorus and carbamate pesticides across a wide concentration range. Pesticides were determined indirectly measuring the enzymatic inhibition effect that they caused. The analysis was based on stopped-flow methodology with a three-step strategy: enzymatic substrate measurement, enzymatic inhibition by pesticide contact with the biosensor, and enzymatic substrate measurement after the inhibition process. The pesticide co... |
| Databáze: | OpenAIRE |
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