Allergen specific responses in cord and adult blood are differentially modulated in the presence of endotoxins

Autor:	Eleonora Dehlink, Zsolt Szépfalusi, Irma Schabussova, Barbara Bohle, E Mayer, Susanne Brix, Vibeke Barkholt, Thomas Eiwegger
Rok vydání:	2008
Předmět:	Allergy Toll-like receptor Lipopolysaccharide Toxin medicine.medical_treatment Immunology Biology medicine.disease_cause medicine.disease Peripheral blood mononuclear cell chemistry.chemical_compound Cytokine Allergen chemistry Cord blood medicine Immunology and Allergy
Zdroj:	Clinical & Experimental Allergy. 38:1627-1634
ISSN:	1365-2222 0954-7894
Popis:	Summary Background Endotoxins are common contaminants in allergen preparations and affect antigen-specific cellular responses. Distinct effects of endotoxin on cells in human umbilical cord and adult blood are poorly defined. Objectives To examine the effect of endotoxins in allergen preparations on cellular responses in human cord and peripheral blood (PB). Methods The endotoxin content in β lactoglobulin (BLG), the peanut allergen Ara h 1 and the major birch pollen allergen Bet v 1 was assessed. Proliferation and cytokine response of mononuclear cells towards contaminated and lipopolysaccharide (LPS)-free allergens were evaluated at different time-points. Fractions of contaminated BLG were generated and assayed on their immuno-stimulatory capacity. The involvement of toll-like receptor (TLR) 2 and 4 was investigated by blocking antibodies and TLR-transfected human embryonic kidney cells. Results The proliferative response of cord blood (CB)-derived mononuclear cells towards allergen-preparations at day 3 was related to the level of LPS contamination. At day 7, proliferation was also detected in the absence of endotoxin. Cytokine production in CB was strongly affected by the content of endotoxin, TLR-4 dependent and not related to the allergen content. Allergen- and endotoxin-induced proliferative responses were generally significantly higher in CB than in adult blood. Conclusion Endotoxins in allergen preparations confound allergen-specific cellular responses. The impact of these contaminations varies with the blood source (CB vs. PB), the type of allergen and is time- and dose-dependent.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8c4de5da863d7c72c6bb4414eaf4f59d https://doi.org/10.1111/j.1365-2222.2008.03080.x Zobrazit plný text záznamu Plný text ve formátu PDF