Regulation by Thyroid Hormone of the Expression of Basement Membrane Components in Rat Prepubertal Sertoli Cells1
| Autor: | Deamaria Piersanti, Emmanuele A. Jannini, F. M. Graziano, Paola Muzi, Salvatore Ulisse, Lucio Gnessi, Piergiuseppe Ceddia, Mario Arizzi, Antonio Pavan, Luisa Cironi, Massimino D'Armiento, Nadia Rucci, Eleonora Carosa |
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| Rok vydání: | 1998 |
| Předmět: | |
| Zdroj: | Endocrinology. 139:741-747 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.139.2.5732 |
| Popis: | The present study reports the modulation of basement membrane (BM) components, laminin, entactin, and type IV collagen, expression in prepubertal rat Sertoli cell by the thyroid hormone T3. Immunocytochemical studies of permeabilized Sertoli cells in culture showed that T3 treatment (10−7 m for 24 h) increased the number of cells staining positive for laminin and/or entactin (from 58 ± 5.3% to 86.4 ± 6.5%, P < 0.01). In contrast, a strong inhibition of type IV collagen immunopositivity was observed. Western blot analysis of Sertoli cell-conditioned media indicated that T3 treatment significantly (P < 0.01) increased the level of secreted entactin by 60–65% without affecting the levels of laminin A and B1/B2 chains. Moreover, thyroid hormone treatment of Sertoli cells significantly reduced type IV collagen secretion by 62% (P < 0.05). Slot blot analysis of poly-A RNA demonstrated a significant (P < 0.01) increase in the level of entactin messenger RNA (mRNA) by 140% (P < 0.01) and a 50% reduction of type I... |
| Databáze: | OpenAIRE |
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