Antidepressive-like effect of a Kampo (traditional Japanese) medicine, kososan (Xiang Su San) in a stress-induced depression-like mouse model: Proteomic analysis of hypothalamus

Autor:	Sari M. Okuda, Haruki Yamada, Tadakazu Maeda, Masamichi Oh-Ishi, Hiroaki Kiyohara, Takayuki Nagai, Toshihiko Hanawa, Yoshio Kodera, Ryoko Hashimoto, Naoki Ito
Rok vydání:	2015
Předmět:	Gel electrophoresis medicine.medical_specialty medicine.diagnostic_test business.industry Kampo Pharmacology 2' 3'-Cyclic-nucleotide 3'-phosphodiesterase Endocrinology Downregulation and upregulation Western blot Hypothalamus Internal medicine medicine Immunohistochemistry Metabotropic glutamate receptor 2 business
Zdroj:	Traditional & Kampo Medicine. 2:50-59
ISSN:	2053-4515
DOI:	10.1002/tkm2.1018
Popis:	Aim A Kampo medicine, kososan (KS), has been used clinically for the treatment of certain depression-like symptoms. Our previous studies using the stress-induced depression-like model mice, showed that oral KS treatment leads to an antidepressive-like effect via the normalization of dysfunction of hypothalamic-pituitary-adrenal axis. In the present study, proteomic analysis was used to identify brain hypothalamus proteins that are affected by KS treatment. Methods KS was orally administered to stress-induced depression-like model mice at 1.0 g/kg/day for 9 days, and the hypothalamus was then analyzed using agarose 2-D gel electrophoresis, followed by mass spectrometry-based protein identification, and then western blot analysis and immunohistochemical analysis. Results The expression of eight proteins was increased or decreased in the hypothalamus of the model mice, but recovered on oral KS treatment. Among them, metabotropic glutamate receptor 2 (mGluR2) and 2′,3′-cyclic nucleotide 3′-phosphodiesterase 1 (CNPase1) were identified as depression-related proteins. Western blot of the hypothalamus confirmed these results and showed that the expression of CNPase2 was opposite to that of CNPase1. On immunohistochemistry, mGluR2 expression in the hypothalamus paraventricular nucleus was downregulated and CNPase2 expression was upregulated in the cerebral fornix in the model mouse, but KS returned them to normal. Conclusion mGluR2 and CNPase in the hypothalamus are associated with the antidepressive-like activity of KS.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8c37f21fed77283084d720aa3dc9948b https://doi.org/10.1002/tkm2.1018 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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