Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing
| Autor: | Jevrosima Stevanovic, Jasna Bosnjak, Miloš Vučićević, Marija Stevanov-Pavlovic, Nevenka Aleksić, Bojan Gajić, Zoran Stanimirovic |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
0303 health sciences biology Alopochen Zoology Milvus milvus General Medicine Sexing biology.organism_classification 010603 evolutionary biology 01 natural sciences Ocyphaps lophotes Cereopsis novaehollandiae 03 medical and health sciences chemistry.chemical_compound chemistry Molecular marker biology.animal Feather visual_art visual_art.visual_art_medium Neophron percnopterus Animal Science and Zoology 030304 developmental biology |
| Zdroj: | Zoo Biology. 32:269-276 |
| ISSN: | 0733-3188 |
| DOI: | 10.1002/zoo.21010 |
| Popis: | The aim of this research was to test the CHD gene (Chromo Helicase DNA-binding gene) as a universal molecular marker for sexing birds of relatively distant species. The CHD gene corresponds to the aim because of its high degree of conservation and different lengths in Z and W chromosomes due to different intron sizes. DNA was isolated from feathers and the amplification of the CHD gene was performed with the following sets of polymerase chain reaction (PCR) primers: 2550F/2718R and P2/P8. Sex determination was attempted in 284 samples of 58 bird species. It was successful in 50 bird species; in 16 of those (Alopochen aegyptiacus, Ara severus, Aratinga acuticaudata, Bucorvus leadbeateri, Cereopsis novaehollandiae, Columba arquatrix, Corvus corax, C. frugilegus, Cyanoliseus patagonus, Guttera plumifera, Lamprotornis superbus, Milvus milvus, Neophron percnopterus, Ocyphaps lophotes, Podiceps cristatus, and Poicephalus senegalus), it was carried out for the first time using molecular markers and PCR. It is reasonable to assume that extensive research is necessary to define the CHD gene as a universal molecular marker for successful sex determination in all bird species (with exception of ratites). The results of this study may largely contribute to the aim. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text