| Popis: |
HBSS/l% BSA, recentrifuged, and resuspended to achieve a final concentration of 0.6 mg of protein/&. Soybean trypsin inhibitor (1 to 15 pg/ml) was added to control or trypsin-treated membranes after the 15-min incubation. We confirmed the ability of the enzymes used to degrade the appropriate substrate by incubating each lipase with a mixture of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin for 15 min at 37 OC. The degradation of these lipids was verified qualitatively by thin layer chromatography on silica gel plates developed first in acetone:petroleum ether (10:30 v/v) (19), then in |
