Titration of numbers of human-derivedMycobacterium lepraerequired to progressively oxidize14C-palmitic acid and release14CO2

Autor:	Shannon Ej, Guebre-Xabier M, Frommel D, Haile-Mariam Hs
Rok vydání:	1994
Předmět:	Lepromatous leprosy biology Albumin General Medicine biology.organism_classification medicine.disease In vitro Microbiology Palmitic acid chemistry.chemical_compound chemistry Catalase medicine biology.protein Titration Mycobacterium leprae Bacteria
Zdroj:	Leprosy Review. 65
ISSN:	0305-7518
DOI:	10.5935/0305-7518.19940009
Popis:	Mycobacterium leprae was isolated from skin-punch biopsies of 2 untreated lepromatous leprosy patients. The bacteria were enumerated, diluted 10-fold and cultured in Middlebrook 7H9 medium supplemented with albumin, dextrose, catalase and 14C-palmitic acid. The cultures were incubated at 33 degrees C in a modified Buddemeyer radiorespiratory detection vessel. Those cultures containing at least 10(7) mycobacteria demonstrated a progressive evolution of 14CO2.
Databáze:	OpenAIRE
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