Measuring Protein Concentration with Absorbance, Lowry, Bradford Coomassie Blue, or the Smith Bicinchoninic Acid Assay Before Electrophoresis
| Autor: | J.P. Dean Goldring |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification 0303 health sciences Chromatography medicine.diagnostic_test 01 natural sciences Absorbance 03 medical and health sciences Electrophoresis Enzyme chemistry 010608 biotechnology Spectrophotometry Coomassie blue Protein purification medicine Bicinchoninic acid assay Protein concentration 030304 developmental biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493987924 |
| DOI: | 10.1007/978-1-4939-8793-1_3 |
| Popis: | Measuring the concentration of proteins is an essential part of enzyme analysis or serves to monitor protein yields and losses during protein isolation procedures. Decisions on the usefulness of any protein isolation procedure depend on knowing the concentration of proteins before and after a procedure. Protein concentration in solution is generally measured with spectrophotometry in the UV range or in the presence of dyes or copper interacting with the protein. This review describes absorbance at 280 nm, the Lowry, Bradford (Coomassie Blue), and Smith (bicinchoninic acid) assays for measuring protein and includes suggestions for optimizing each method. |
| Databáze: | OpenAIRE |
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