Abstract TP334: A Novel Murine Model of the Effects of Helicopter Emergency Medical Services Transportation on Ischemia and Thrombolysis
| Autor: | Nirav Dhanesha, Thomas Schnell, Jonathan DeShaw, Bradley Parker, Anil K. Chauhan, Salam Rahmatalla, Andrew A. Pieper, Enrique C. Leira |
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| Rok vydání: | 2019 |
| Předmět: |
Advanced and Specialized Nursing
medicine.medical_specialty business.industry medicine.medical_treatment Ischemia Thrombolysis medicine.disease Mechanical thrombectomy Medical services Embolism Murine model Emergency medicine Emergency medical services Medicine Neurology (clinical) Cardiology and Cardiovascular Medicine business Stroke |
| Zdroj: | Stroke. 50 |
| ISSN: | 1524-4628 0039-2499 |
| DOI: | 10.1161/str.50.suppl_1.tp334 |
| Popis: | Background: Helicopter emergent medical services (HEMS) transportation is critical for rapid transportation of patients for mechanical thrombectomy and rtPA-mediated reperfusion. However, the effect of the multiple unique physical factors present in HEMS on the outcomes of these procedures is not known. Experimental models of ischemia-reperfusion that replicate HEMS conditions are needed in order to address this issue before putative neuroprotective therapies during flight trials can be accurately evaluated. Methods: A novel experimental model of “drip & ship” during HEMS was developed using middle cerebral artery occlusion (MCAO) with autologous clot in mice and an Mi2 helicopter adapted for animal research. Since vibration is one of the most salient physical components in HEMS, we isolated this factor for mechanistic investigation. The vibratory signature during HEMS was recorded in-flight using accelerometers and then recapitulated in a six-degree-of-freedom man-rated Moog-FCS motion platform in the laboratory. We evaluated two approaches (simultaneous vs. sequential) to expose mice with MCAO to the three different settings (helicopter/simulator/ground) in order to find the most optimal methodologically. Results: A clot length of 30 mm and 90 min of rTPA initiation post MCAO achieved significant infarctions while minimizing periprocedural mortality. Two MCAOs were the maximum capable of achieving occlusion times Conclusion: We have developed an animal model to study the effects of HEMS on rtPA/reperfusion. An ongoing experiment measuring infarction volume and clinical outcomes will determine the need to maintain an actual HEMS for future experiments, or whether a laboratory simulator will suffice. |
| Databáze: | OpenAIRE |
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