Hypoxia Inducible Factor 1: A Urinary Biomarker of Kidney Disease
| Autor: | Kim Vo, Dominic S. Raj, R Rahman, S Movafagh, M Sanaei-Ardekani, D Bhatia, Mahta Mahmoudieh, E H Kim, Arthur F. Harralson |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Kidney business.industry General Neuroscience Urinary system 030232 urology & nephrology Kidney metabolism General Medicine In situ hybridization Disease Urine medicine.disease General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Endocrinology Internal medicine medicine Biomarker (medicine) General Pharmacology Toxicology and Pharmaceutics business Kidney disease |
| Zdroj: | Clinical and Translational Science. 10:201-207 |
| ISSN: | 1752-8054 |
| DOI: | 10.1111/cts.12445 |
| Popis: | Identifying noninvasive biomarkers of kidney disease is valuable for diagnostic and therapeutic purposes. Hypoxia inducible factor 1 (HIF-1) expression is known to be elevated in the kidneys in several renal disease pathologies. We hypothesized that the urinary HIF-1a mRNA level may be a suitable biomarker for expression of this protein in chronic kidney disease (CKD). We compared HIF-1a mRNA levels from urine pellets of CKD and healthy subjects. To ensure that urinary HIF-1a mRNA is of kidney origin, we examined colocalization of HIF-1a mRNA with two kidney specific markers in urine cells. We found that HIF-1a mRNA is readily quantifiable in urine pellets and its expression was significantly higher in CKD patients compared with healthy adults. We also showed that the urinary HIF-1a mRNA comes primarily from cells of renal origin. Our data suggest that urinary HIF-1a mRNA is a potential biomarker in CKD and can be noninvasively assessed in patients. |
| Databáze: | OpenAIRE |
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