The Electrical Property of SiO2/Graphene/PBA-NHS/anti O and K E. coli Antibodies as Sensing Layer for Escherichia coli Bacteria Sensor
| Autor: | Royan, Atqiya Muslihati, Nurliyana Mad Rosni, G. Romadhona, K. M. Wibowo, Mohd Zainizan Sahdan, Fatiatun |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | IOP Conference Series: Materials Science and Engineering. 982:012024 |
| ISSN: | 1757-899X 1757-8981 |
| Popis: | This paper explains the effects of BSA/PBA-NHS/Graphene/SiO2 used as a sensing layer for E. coli bacteria sensor based on the electrical properties. In this research, graphene is used as a sensing layer due to its superior properties especially its biocompatibility. The biocompatibility of graphene was examined by topological properties by AFM. Moreover, it was also confirmed by Raman spectroscopy. The topological structure and Raman spectroscopy showed good results which is indicate the graphene layer suitable for biosensing applications. Additionally, it was also confirmed that the antibody was successfully immobilized on the modified SiO2/graphene/PBANHS. PBANHS is used as a linker due to its cost-effective and time-saving. The electrical properties of the sensing layer before and after the immobilized antibody shows a significant difference. When the number of E. coli bacteria increases, the current increase also became higher. The change in resistance gradually increases from 0.04 to 2.62 Ħ when the number of bacteria increases from 55 to 1200 CFU/ml.-+. |
| Databáze: | OpenAIRE |
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