Autor: Christine Sercombe, Kamel Kacem, Richard Sercombe, Jacques Seylaz, Philippe Gomis
Rok vydání: 2000
Předmět:
Zdroj: The Histochemical Journal. 32:673-678
ISSN: 0018-2214
DOI: 10.1023/a:1004115432660
Popis: Our objective was to determine whether subarachnoid haemorrhage modifies cerebral artery smooth muscle cell phenotype and the contractile protein α-actin measured 7 days after haemorrhage. We used a rabbit subarachnoid haemorrhage model and immunofluorescence labelling of α-smooth muscle actin, vimentin and desmin. The paired comparison between the haemorrhage and sham rabbits was performed using confocal laser-scanning microscopy. We found in the haemorrhage group significantly less intense α-actin immunostaining (p = 0.036) and more intense vimentin immunostaining (p = 0.043) but no significant change in the intensity of desmin staining. Our results indicate an absolute decrease after subarachnoid haemorrhage in the amount of functional α-actin and in the light of the literature may suggest a certain degree of dedifferentiation of smooth muscle cells in the cerebral artery wall.
Databáze: OpenAIRE