Identification and Kinetic Characteristics of an Indigenous Diesel-degrading Gordonia alkanivorans Strain

Autor: Chiu-Chung Young, Mao Song Yeh, Fo-Ting Shen, Jo Shu Chang, Ta Chen Lin
Rok vydání: 2005
Předmět:
Zdroj: World Journal of Microbiology and Biotechnology. 21:1409-1414
ISSN: 1573-0972
0959-3993
DOI: 10.1007/s11274-005-5742-7
Popis: An indigenous strain Gordonia alkanivorans CC-JG39 was isolated from oil-contaminated sludge of a local gas station located in central Taiwan. The bacterial isolate was able to grow on diesel-containing Bushnell–Haas medium and also tolerate various chemical additives frequently used in petroleum products (e.g. BETX, methyl-tert-butyl ether, and naphthalene). Kinetics of diesel-limited cell growth and biodegradation of diesel followed a Monod-type model. The kinetic constants for cell growth (μmax and KS,G) were 0.158 h−1 and 3196 mg/l, respectively, while those for biodegradation of diesel (vmax, diesel and KS,D) were 3.59 mg/h/mg cell and 2874 mg/l, respectively. G. alkanivorans CC-JG39 produced extracellular surface-active material, leading to a low surface tension of nearly 33 mN/m. The CC-JG39 strain also possessed the ability to float towards the oil/water interface. These features might play some roles in enhancing the mass transfer efficiency between oil substrate and the bacterial cells. Therefore, G. alkanivorans CC-JG39 may have potential applications in bioremediation of oil pollution sites.
Databáze: OpenAIRE