Use of the 'Acetylene blockage' technique for assaying denitrification in a salt marsh
Autor: | C. D. Van Raalte, D. G. Patriquin |
---|---|
Rok vydání: | 1979 |
Předmět: |
geography
animal structures Denitrification geography.geographical_feature_category Ecology fungi Growing season Sediment Aquatic Science Biology equipment and supplies Spartina alterniflora biology.organism_classification chemistry.chemical_compound Acetylene chemistry Nitrate Salt marsh Environmental chemistry embryonic structures Botany Gas chromatography Ecology Evolution Behavior and Systematics |
Zdroj: | Marine Biology. 52:315-320 |
ISSN: | 1432-1793 0025-3162 |
DOI: | 10.1007/bf00389072 |
Popis: | The “acetylene blockage” technique was evaluated for measurement of denitrification in salt-marsh sediments (near Halifax, Nova Scotia, Canada). N2O in the gas phase of closed Spartina alterniflora marsh-sediment systems was analyzed with use of a thermal conductivity gas chromatograph sensitive to approximately 0.1 nmoles ml-1 gas. No N2O was detected for unfertilized sediment samples taken through the growing season and incubated in sealed buckets with 10% C2H2. For sediment samples amended with nitrate and for enrichments, initial rates of N2O evolution were higher in the presence of 10% C2H2 than in the absence of C2H2, but after longterm incubation N2O was consumed in some samples containing C2H2 as well as in samples without C2H2. In addition, total gaseous nitrogen (N2 and N2O) production in the absence of C2H2 was higher than in the presence of C2H2. Acetylene appears to be an inconsistent inhibitor of N2O reduction in salt-marsh sediments. The usefulness of the acetylene-denitrification technique in this habitat is, therefore, questionable. |
Databáze: | OpenAIRE |
Externí odkaz: |