Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage

Autor:	Sivakumar Pattathil, Robert G. Brannan, Ryan Goelz, Ahmed Faik
Rok vydání:	2019
Předmět:	Glycan biology Chemistry Asimina General Chemical Engineering food and beverages biology.organism_classification Polyphenol oxidase Glycome Industrial and Manufacturing Engineering Pascalization Cell wall Postharvest biology.protein Browning Food science Food Science
Zdroj:	Food Science and Technology International. 25:711-722
ISSN:	1532-1738 1082-0132
DOI:	10.1177/1082013219856769
Popis:	This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw ( Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage. We hypothesize that high pressure processing may inhibit enzymatic action responsible for pawpaw's deleterious postharvest tissue softening and browning. Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition. Results show that both varieties have typical type I primary cell walls of flowering dicots. However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls. High pressure processing treatment does not seem to be effective at eliminating polyphenol oxidase activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8bbdb450d415621b6d0ffb27db7126c7 https://doi.org/10.1177/1082013219856769 Zobrazit plný text záznamu