Effects of Androgen on Androgen Receptor Expression in Rat Testicular and Epididymal Cells: A Quantitative Immunohistochemical Study1
| Autor: | Ilpo Huhtaniemi, Matthew P. Hardy, Alfred J. Moo-Young, C. Wayne Bardin, Li-Ji Zhu, Ivan V. Inigo |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Leydig cell medicine.drug_class Cell Biology General Medicine Biology Testicle urologic and male genital diseases Androgen Epididymis Sertoli cell Androgen receptor medicine.anatomical_structure Endocrinology Reproductive Medicine Internal medicine medicine Spermatogenesis Testosterone |
| Zdroj: | Biology of Reproduction. 63:368-376 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod63.2.368 |
| Popis: | Androgen is essential for maintenance of spermatogenesis in the testis and for maturation of spermatozoa in the epididymis. The effects of androgen are mediated through its receptor (AR), the levels of which are, in turn, regulated by androgen. Previous studies have shown that AR concentrations in Leydig and Sertoli cells are differentially regulated during development. The aim of the present study was to determine if cell-type-specific regulation of AR by androgen occurs in testicular and epididymal cells during adulthood. Adult male rats were treated with the LHRH-antagonist Azaline B (100 g/day) by osmotic pump for 1, 2, 3, 4, or 8 wk to suppress endogenous androgen, with identical numbers of intact control animals at each time period. An androgen replacement group was simultaneously treated with the antagonist and a synthetic androgen, 7α-methyl-19-nortestosterone (MENT), during the final 4 wk of the experiment. Levels of nuclear AR protein in specific cell types were quantified by immunohist... |
| Databáze: | OpenAIRE |
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