Seroprevalence of Rickettsia spp. in Equids and Molecular Detection of ‘Candidatus Rickettsia amblyommii’ in Amblyomma cajennense Sensu Lato Ticks From the Pantanal Region of Mato Grosso, Brazil
| Autor: | Andréla L. T. Melo, Daniel Moura de Aguiar, Alvair da S. Alves, Lucas Gaíva E Silva, Marcelo Bahia Labruna, Richard de Campos Pacheco, Thiago F. Martins, Alice Mamede Costa Marques Borges, Marcus Vinicius de Amorim |
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| Rok vydání: | 2014 |
| Předmět: |
Veterinary medicine
animal structures General Veterinary biology Amblyomma bacterial infections and mycoses biology.organism_classification Rickettsia rickettsii Amblyomma cajennense law.invention Titer Infectious Diseases Rickettsia law Insect Science parasitic diseases bacteria Seroprevalence Parasitology Nymph Polymerase chain reaction |
| Zdroj: | Journal of Medical Entomology. 51:1242-1247 |
| ISSN: | 0022-2585 |
| Popis: | The aim of the study was to evaluate exposure of equids to rickettsial agents (Rickettsia rickettsii, Rickettsia parkeri, 'Candidatus Rickettsia amblyommii', Rickettsia rhipicephali, and Rickettsia bellii) and rickettsial infection in ticks of a Pantanal region of Brazil. Sera of 547 equids (500 horses and 47 donkeys) were evaluated by indirect immunofluorescence assay. In total, 665 adults and 106 nymphal pools of Amblyomma cajennense F. sensu lato, 10 Dermacentor nitens Neumann ticks, and 88 larval pools of Amblyomma sp. were tested by polymerase chain reaction (PCR). Overall, 337 (61.6%) equids were reactive (titer ≥64) to at least one antigen of Rickettsia spp. The prevalence values for Rickettsia were 66%, and the highest endpoint titers were observed for 'Ca. R. amblyommii'. By PCR 3 (0.45%) A. cajennense s.l. females were positive for 'Ca. R. amblyommii'. Minimum infection rates of 0.75% for nymphs and 0.34% for larvae were calculated. Positive samples of ticks have had a fragment of the 16S mitochondrial rRNA gene sequenced and sequences showed 99% identity to Amblyomma sculptum Berlese. This study reports a wide exposure of equids to Rickettsia agents, and PCR evidence of infection with 'Ca. R. amblyommii', for the first time, in A. sculptum. |
| Databáze: | OpenAIRE |
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