Studies on the Hydrolytic Condition of β‐1,3 Glucan from Yeast by Fluorophore‐Assisted Carbohydrate Electrophoresis
| Autor: | Gang-Liang Huang, Xin-Ya Mei, Man-Xi Liu |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Gel electrophoresis
chemistry.chemical_classification Chromatography Biochemistry (medical) Clinical Biochemistry Fluorescence spectrometry Disaccharide Biochemistry Analytical Chemistry Hydrolysis chemistry.chemical_compound chemistry Electrochemistry Tetrasaccharide Fluorophore-assisted carbohydrate electrophoresis Polyacrylamide gel electrophoresis Spectroscopy Glucan |
| Zdroj: | Analytical Letters. 38:477-485 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1081/al-200047797 |
| Popis: | Fluorophore‐assisted carbohydrate electrophoresis (FACE) is a straightforward, sensitive method for determining the presence and relative abundance of individual (oligo)saccharide in a(n) (oligo)saccharide mixture. The single‐terminal aldehydes of oligoglucoside residues released by acid hydrolysis of β‐1,3 glucan from yeast were tagged with the charged fluorophore 8‐aminonaphthalene‐1,3,6‐trisulfonate (ANTS) and separated with high resolution on the basis of size by polyacrylamide gel electrophoresis. ANTS fluorescence labeling was not biased by oligoglucoside length; therefore, band fluorescence intensity was directly related to the relative abundance of individual oligoglucoside moieties in heterogeneous samples. FACE analysis revealed that the major oligoglucoside mixture released by acid hydrolysis from β‐1,3 glucan was composed of monosaccharide, disaccharide, trisaccharide, and tetrasaccharide, respectively. And the best hydrolytic condition is at a concentration of 2.0 mol/L TFA, heated a... |
| Databáze: | OpenAIRE |
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