| Autor: |
Erika Mekis, György Béndek, György Pintér |
| Rok vydání: |
1987 |
| Předmět: |
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| Zdroj: |
Journal of the Institute of Brewing. 93:396-398 |
| ISSN: |
0046-9750 |
| Popis: |
A simple system has been constructed for the quantitative determination of barley β-glucans. Measurements were made by the flow-injection-analysis (FIA) technique using the fluorescent dye Calcofluor as a specific reagent for (1·3) (1·4)–B—D—linkages. In this paper a single-line system is described involving only one pump samples of β-glucan being directly injected into the reagent stream. The reaction results in an increased intensity of fluorescence detected by a flow-through-cell fluorimeter and evaluated as peak heights or peak areas by a printer-plotter. The analysis is completed within 20s. The operational parameters were set during an optimization procedure. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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