Comparison of methods for the detection of Neisseria gonorrhoeae from South African women attending antenatal care
| Autor: | Partson Tinarwo, Glynis Oree, Nathlee Abbai, Hopolang C Maise, Meleshni Naicker, Veron Ramsuran |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
030306 microbiology business.industry Moderate level Public Health Environmental and Occupational Health Diagnostic accuracy Dermatology Ribosomal RNA 16S ribosomal RNA medicine.disease_cause Virology 03 medical and health sciences 0302 clinical medicine Infectious Diseases Real-time polymerase chain reaction TaqMan Neisseria gonorrhoeae Medicine Pharmacology (medical) 030212 general & internal medicine business |
| Zdroj: | International Journal of STD & AIDS. 32:396-402 |
| ISSN: | 1758-1052 0956-4624 |
| Popis: | The detection of Neisseria gonorrhoeae using culture assays is challenging. This study aims to compare different assays for the detection of N. gonorrhoeae. This cross-sectional study was conducted at King Edward VIII Hospital and included 307 antenatal attendees, each willing to provide two endocervical swabs. The first swab was used for culture identification of N. gonorrhoeae, and the second swab was processed for the detection of the pathogen by the TaqMan quantitative polymerase chain reaction (qPCR) assay, an in-house 16S ribosomal RNA ( rRNA) PCR and PCR detection of the opa gene. Culture and the nucleic acid amplification assays were each used as comparator tests in the analysis. Sensitivity and specificity were calculated using RS Studio. The prevalence of N. gonorrhoeae was 7.8%. When compared to the TaqMan assay, the 16S rRNA PCR exhibited the highest sensitivity of 62%, with a substantial level of agreement (kappa level of agreement: 0.60), followed by the opa PCR (38%) with a moderate level of agreement (0.52) and culture exhibiting the lowest sensitivity of 25% with a fair level of agreement (0.38). The diagnostic accuracy of all the assays was >90%. The TaqMan qPCR assay has the ability to serve as a future diagnostic assay for the detection of N. gonorrhoeae. |
| Databáze: | OpenAIRE |
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