CaMKII Mediates TGFβ1-Induced Fibroblasts Activation and Its Cross Talk with Colon Cancer Cells
| Autor: | Wei Chen, Juan Su, Jun Zhang, Jian Kang, Wen Ai, He-Sheng Luo, Yanwei Chen, Ping An, Yijuan Ding |
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| Rok vydání: | 2021 |
| Předmět: |
MAPK/ERK pathway
Stromal cell Physiology Colorectal cancer Chemistry musculoskeletal neural and ocular physiology Gastroenterology medicine.disease environment and public health Small hairpin RNA 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure nervous system Tumor progression 030220 oncology & carcinogenesis Ca2+/calmodulin-dependent protein kinase cardiovascular system Cancer research medicine 030211 gastroenterology & hepatology biological phenomena cell phenomena and immunity Fibroblast Protein kinase B |
| Zdroj: | Digestive Diseases and Sciences. 67:134-145 |
| ISSN: | 1573-2568 0163-2116 |
| DOI: | 10.1007/s10620-021-06847-0 |
| Popis: | Cancer-associated fibroblasts (CAFs), as the activated fibroblasts in tumor stroma, are important modifiers of tumor progression. TGFβ1 has been the mostly accepted factor to fuel normal fibroblasts transformation into CAFs. Ca2+/calmodulin-dependent protein kinase II (CaMKII) is thought to play an important role in fibroblasts activation induced by TGFβ1. The aim of this study is to investigate the potential role of CaMKII in TGFβ1-induced fibroblasts activation and CAF-like differentiation. Cross talk between CaMKII-dependent fibroblasts and colon cancer in colon cancer progression also was addressed Immunostaining demonstrated that in colon cancer stroma, CaMKII overexpressed in stromal CAFs. In vitro, TGFβ1 increased CAF markers expression in human colon fibroblasts CCD-18Co, but not in CaMKII depletion fibroblasts. CaMKII knockdown by CaMKII shRNA significantly inhibited TGFβ1-induced fibroblasts activation and CAF-like differentiation. Smad3, AKT, and MAPK were targeted in TGFβ1–CaMKII-mediated pathway. Human colon cancer cell line HCT-116 activated fibroblasts directly, whereas CaMKII depletion dragged CCD-18Co fibroblasts undergoing CAF-associated trans-differentiation. Furthermore, increased proliferation, migration, and invasion of colon cancer cells were stimulated when co-cultured with normal fibroblasts, but not with CaMKII depletion fibroblasts. These findings provide evidence that CaMKII is a critical mediator in TGFβ1-induced fibroblasts activation and is involved in the cross talk with colon cancer cells. CaMKII is a potentially effective target for future treatment of colon cancer. |
| Databáze: | OpenAIRE |
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