Molecular characterization, structure prediction and insilico analysis of hydrocarbon degrading surfactin synthetase from marine sponge-associated Bacillus licheniformis NIOT-06
| Autor: | Balakrishnan Meena, Lawrance Anburajan, Nambali Valsalan Vinithkumar, Gopal Dharani, Ramalingam Kirubagaran, Rangamaran Vijaya Raghavan, Toms C. Joseph |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Expression vector Operon 030106 microbiology Biology medicine.disease_cause biology.organism_classification law.invention 03 medical and health sciences Open reading frame chemistry.chemical_compound 030104 developmental biology Biochemistry chemistry law Genetics medicine Recombinant DNA lipids (amino acids peptides and proteins) Bacillus licheniformis Surfactin Polyacrylamide gel electrophoresis Escherichia coli |
| Zdroj: | Gene Reports. 5:40-44 |
| ISSN: | 2452-0144 |
| DOI: | 10.1016/j.genrep.2016.08.006 |
| Popis: | An open reading frame encoding the surfactin synthetase gene (srfA) was cloned from the Bacillus licheniformis NIOT-06 genome. An expression plasmid containing the srfA operon was introduced into Escherichia coli M15 cells and the recombinant biosurfactant was purified and quantified. Purified recombinant surfactin revealed excellent emulsification activity. HPLC fraction of the purified recombinant surfactin revealed significant surface tension reducing property. Secondary structure of the surfactin synthetase enzyme was predicted to have alpha-helical structure. Three dimensional structure model of surfactin synthase was found to be 0.243 A units. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect