| Autor: |
Jose Requejo-Isidro, Dylan M. Owen, James McGinty, Mark A. A. Neil, Hugh B. Manning, Christopher Dunsby, Ian Munro, M. Jon Lever, Pieter A.A. De Beule, Richard K.P. Benninger, Neil Galletly, Praveen Anand, Paul M. W. French, Gordon Stamp, Clifford Talbot, Daniel S. Elson |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
SPIE Proceedings. |
| ISSN: |
0277-786X |
| DOI: |
10.1117/12.700081 |
| Popis: |
We present the design, characterization and application of a novel, rapid, optically sectioned hyperspectral fluorescence lifetime imaging (FLIM) microscope. The system is based on a line scanning confocal configuration and uses a highspeed time-gated detector to extract lifetime information from many pixels in parallel. This allows the full spectraltemporal profiles of a fluorescence decay to be obtained from every pixel in an image. Line illumination and slit detection also gives the microscope a confocal optical sectioning ability. The system is applied to test samples and unstained biological tissue. In future, this microscope will be combined with recently-developed continuously electronically tunable, pulsed light sources based on tapered, micro-structured optical fibers. This will allow hyperspectral FLIM to be combined with the advantages of excitation spectroscopy to gain further insight into complex biological specimens including tissue and live cell imaging. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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