Nutrition effects on the biofilm immobilization and 3,5-DNBA degradation ofComamonas testosteroniA3 during bioaugmentation treatment

Autor: Cheng Zhongqin, Mengying Li, Hongqing Xu, Xiangxun Meng, Liqun Xie
Rok vydání: 2014
Předmět:
Zdroj: Biotechnology and Applied Biochemistry. 62:719-725
ISSN: 0885-4513
DOI: 10.1002/bab.1314
Popis: The survival of inoculated microbes is critical for successful bioaugmentation in wastewater treatment. The influence of readily available nutrients (RANs) on the colonization of two functional bacteria, Pseudomonas putida M9, a strong biofilm-forming strain, and Comamonas testosteroni A3, a 3,5-dinitrobenzoic acid (3,5-DNBA)-degrading strain, in biofilms was studied with 3,5-dinitrobenoic acid synthetic wastewater (DCMM) complemented with various ratios of Luria-Bertani broth (LB). With the increase in LB rate, the biofilm biomass was increased, the percentage of gfp-labeled M9 measured in the mixed culture enhanced, and also M9 became dominant. In laboratory-scale sequencing batch biofilm reactors, with the increase in 3,5-DNBA concentration and extension of the running time, the 3,5-DNBA removal in DCMM wastewater complemented with RANs tended to be more efficient and its removal rates increased gradually over the experimental period. Our study demonstrated that supplementing RANs could be a useful strategy for enhancing colonization of degrading bacteria in wastewater treatment systems.
Databáze: OpenAIRE