Detection of verocytotoxin-producing Escherichia coli (VTEC) in minced beef and raw milk by colony blot hybridization
| Autor: | G. Normanno, Angela Miccolupo, Angela Dambrosio, Antonio Parisi, G. Santagada, Carmine Pedarra |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
food and beverages
Verocytotoxin Raw milk Biology medicine.disease_cause Minced beef food.food Microbiology chemistry.chemical_compound fluids and secretions food chemistry STX2 VTEC Multiplex polymerase chain reaction medicine Food science Escherichia coli Food Science Biotechnology Food contaminant |
| Zdroj: | Food Control. 21:770-773 |
| ISSN: | 0956-7135 |
| DOI: | 10.1016/j.foodcont.2009.09.003 |
| Popis: | Verocytotoxin-producing Escherichia coli (VTEC) are foodborne pathogens that cause outbreaks linked to consumption of meat and raw milk. In this note the authors report results obtained from a survey conducted on minced beef and raw bovine milk samples using a Multiplex PCR (M-PCR) for the detection of eae, stx1, stx2 and hlyA genes as a screening step followed by a colony blot hybridization (CBH) technique for the isolation of the VTEC. Of 100 minced beef and 123 raw milk samples, 13 (13%) and 7 (5.7%) were positive in the M-PCR and among these 9 and 3 strains were isolated using CBH, respectively. All isolates showed the presence of the stx2 gene, single or in association with the other investigated genes. None of the isolates belonged to the O157, O26, O91, O103, O111 and O145 serogroups. The study showed that the use of M-PCR for the screening of samples coupled with a sensitive and specific detection technique, could improve the possibility of detection of VTEC strains in foods. Moreover, the presence of VTEC in minced beef and in raw milk confirms their important role as putative vehicles of infection to humans. Stringent control of these foodstuffs is essential for food safety purposes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect