Proliferative Activity of Megakaryocytes in Myelo- and Lymphoproliferative Diseases
| Autor: | Malgorzata Sidor, Krzysztof Gawronski, Kazimierz Sulek, Maria Domagala |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty biology business.industry Essential thrombocythemia Myelodysplastic syndromes Immunology Myeloid leukemia Cell Biology Hematology medicine.disease Biochemistry Polycythemia vera medicine.anatomical_structure Ki-67 medicine biology.protein Bone marrow Myelofibrosis business Multiple myeloma |
| Zdroj: | Blood. 106:4954-4954 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v106.11.4954.4954 |
| Popis: | Aim of the study: Evaluation of significance of investigation of Ki67 and AgNOR staining as an indices of proliferative activity of megakaryocytes. Material and methods: Bone marrow smears from 151 patients suffering from hematological malignancies: polycythemia vera (PV) − 14 cases, myelofibrosis (MF) − 14, essential thrombocythemia (ET) − 15, chronic myeloid leukemia (CML) − 11, myelodysplastic syndromes (MDS) − 18; acute myeloid leukemia (AML) − 30, acute lymphoblastic leukemia (ALL) − 10, low grade non-Hodgkin’s lymphomas (NHL) with bone marrow infiltration − 20, multiple myeloma (MM) − 19. Cytological slides were stored in the room temperature more than 24 hours and then stained using immunocytochemical method for Ki67 and cytochemical method for AgNOR. Slides were analyzed using illuminating microscope with digital image analysis (software LUCIA). Mean particles area, mean nuclear area and maturity of nucleus in AgNOR staining as well as intensity of Ki67 expression were evaluated, according to arbitrary scale (strong, medium, light, no reaction). Results: Look at the table 1 and table 2. Conclusions:There is a good correlation between expression of Ki-67 and AgNOR’s in megakaryocytes as well as between nuclear maturity and indices of Ki-67 and AgNOR in myeloproliferative disorders. Differences in AgNOR’s and Ki 67 staining area between myeloproliferative diseases and another hematological malignancies are also significant. Table 1: Values of indices of the Ki67 and AgNOR in megakaryocytes Diseases Mean nucleus surface μm2 Mean AgNOR particle area in nucleus of megakaryocytes μm2 Mean Ki67 particle area in nucleus of megakaryocytes μm2 Intensity of Ki67 expression in megakaryocytes Grade of megakaryocytes maturity PV 667,29 0,68 0,19 Medium III and IV ET 812,32 0,82 0,29 Strong / Medium III and IV MF 767,92 0,55 0,26 Strong / Medium III / IV CML 734,30 0,57 0,15 Medium / Light III / IV MDS 645,67 0,06 0,03 Light III / IV AML 679,04 0,04 No reaction No reaction IV ALL 712,23 0,03 No reaction No reaction IV NHL 798,56 0,04 No reaction No reaction IV MM 629,49 0,04 No reaction No reaction IV Table 2: Intensity of Ki67 expression in megakaryocytes in hematological disorders Diseases Strong – % of cells Medium – % of cells Light – % of cells No reaction – % of cells PV 36 43 14 7 ET 56 33 11 0 MF 51 32 16 1 CML 16 43 34 7 MDS 9 12 68 11 AML 0 0 0 0 ALL 0 0 0 0 NHL 0 0 0 0 MM 0 0 0 0 |
| Databáze: | OpenAIRE |
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