| Autor: |
Charles K. Pallaghy, Gottfried Wagner, Roland Rossbacher |
| Rok vydání: |
1984 |
| Předmět: |
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| Zdroj: |
Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 3:664-666 |
| ISSN: |
0168-583X |
| Popis: |
X-ray microanalysis, widely used for ionic detection in cell tissues, was performed on 0.5 μm thick glutaraldehyde-osmiumtetroxide fixed cell sections. Calcium in a water-insoluble state was found in membrane bound vesicles of about 0.5 μm in diameter. No other cell compartment showed a comparable calcium accumulation. In vivo staining by the fluorescent calcium indicator dye chlorotetracycline revealed a vesicular calcium pattern in Mougeotia matching the results by X-ray microanalysis: a dense population of calcium vesicles is located close to the chloroplast edge where the actin filaments are attached. The data find further support from X-ray microanalysis of shock-frozen hydrated Mougeotia cells and of the closely related green algal cells Spirogyra and Zygnema (Zygnemaceae). X-ray microanalysis as performed in this study seems a reliable tool for the detection of water-insoluble calcium stored by membrane-covered cytoplasmic compartments. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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