Separation and characterization of peanut phospholipid molecular species using high-performance liquid chromatography and fast atom bombardment mass spectrometry
| Autor: | Carol A. Haney, J. A. Singleton, J. H. Sanford, Larry F. Stikeleather, M. Ruan |
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| Rok vydání: | 1999 |
| Předmět: |
Phosphatidylethanolamine
Chromatography Elution General Chemical Engineering Organic Chemistry Phospholipid food and beverages Fraction (chemistry) Reversed-phase chromatography Fast atom bombardment Mass spectrometry High-performance liquid chromatography chemistry.chemical_compound chemistry lipids (amino acids peptides and proteins) |
| Zdroj: | Journal of the American Oil Chemists' Society. 76:49-56 |
| ISSN: | 0003-021X |
| DOI: | 10.1007/s11746-999-0046-y |
| Popis: | Total lipid extracts from peanut seed were separated on a silica column into a triacylglycerol fraction and a polar lipid fraction by high-performance liquid chromatography (HPLC). The polar fraction containing the phospholipids was retained on the precolumn, and the triacylglycerol fraction was eluted to a waste flask by a special valve arrangement. Phospholipids were eluted from the precolumn and separated into various classes on a silica analytical column. Each phospholipid class was manually collected and subsequently subjected to reversed-phase HPLC in tandem with a fast atom bombardment mass spectrometer. Phosphatidylethanolamine was separated into five molecular species. Phosphatidylinositol and phosphatidylcholine were each separated into six molecular species. |
| Databáze: | OpenAIRE |
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