Impact of a G2-EPSPS & GAT Dual Transgenic Glyphosate-Resistant Soybean Line on the Soil Microbial Community under Field Conditions Affected by Glyphosate Application
| Autor: | Yong-Hua Yang, Xiao-Mei Hua, Rong-Wu Yang, Tongming Yin, Minkai Yang, Xinhong Xu, Zhi Hong, Jin-Liang Qi, Zhongling Wen, Aliya Fazal, Gui-Hua Lu |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
biology 030306 microbiology Transgene food and beverages Soil Science Plant Science General Medicine 16S ribosomal RNA biology.organism_classification 03 medical and health sciences Horticulture chemistry.chemical_compound Microbial population biology chemistry Seedling Glyphosate Gene Relative species abundance Ecology Evolution Behavior and Systematics Bradyrhizobium elkanii 030304 developmental biology |
| Zdroj: | Microbes and Environments. 35 |
| ISSN: | 1347-4405 1342-6311 |
| DOI: | 10.1264/jsme2.me20056 |
| Popis: | In the past thirty years, the biosafety of the aboveground part of crops, including horizontal gene transferal through pollen dispersal and hybridization, has been the focus of research; however, microbial communities in the underground part are attracting increasing attention. In the present study, the soybean root-associated bacterial communities of the G2-EPSPS plus GAT transgenic soybean line Z106, its recipient variety ZH10, and Z106 treated with glyphosate (Z106J) were compared at the seedling, flowering, and seed filling stages by high-throughput sequencing of the V4 hypervariable regions of 16S rRNA gene amplicons using Illumina MiSeq. The results obtained showed no significant differences in the alpha/beta diversities of root-associated bacterial communities at the three stages among ZH10, Z106, and Z106J under field growth conditions; however, the relative abundance of four main nitrogen-fixing bacterial genera significantly differed among ZH10, Z106, and Z106J. Ternary plot results indicated that in the root compartment, the proportional contributions of rhizobial nitrogen-fixing Ensifer fredii and Bradyrhizobium elkanii, which exhibit an extremely broad nodulation host range, markedly differed among the three treatments at the three stages. Thus, the present results indicate that transgenic G2-EPSPS and GAT soybean may induce different changes in functional bacterial species in soil, such as E. fredii and B. elkanii, from ZH10, which were compensated for/enriched at the flowering and seed filling stages, respectively, to some extent through as of yet unknown mechanisms by transgenic soybean treated with glyphosate. |
| Databáze: | OpenAIRE |
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