Determination of amount of retinylpalmitate and ascorbic acid of extract gel of sweet corn fibre by UVand HPTLC method

Autor: N Sunitha, S Manohar Babu, B Appa Rao
Rok vydání: 2021
Zdroj: IP International Journal of Comprehensive and Advanced Pharmacology. 6:201-204
ISSN: 2456-9542
2581-5555
Popis: Sweet corn fibres of about 15 gm were extracted with methanol for 5 hours in heating mantle at 40C and filtered and allowed to dry. The dried gel was further analyzed for estimation of Retinyl palmitate by spectrophotometrically by laboratory method and found to be 140 mg/kg. The dried extract gel was further estimated for ascorbic acid both by UV and HPTLC and found to be linear in the range of 1-5 ug/ml and 5-10ug/ml, correlation coefficient was found to be 0.997 and 0.998 and the amount of ascorbic acid was found to be 338 ng/ml and 9.9 ng/ml by UV and HPTLC respectively. The method was found to be linear.
Databáze: OpenAIRE