Further Evidence That the Majority of Primary Nuclear RNA Transcripts in Mammalian Cells Do Not Contribute to mRNA

Autor:	James E. Darnell, M. Salditt-Georgieff
Rok vydání:	1982
Předmět:	Cloning chemistry.chemical_classification Messenger RNA Chinese hamster ovary cell RNA Cell Biology Biology Molecular cloning Molecular biology Biochemistry chemistry Complementary DNA Nucleic acid Nucleotide Molecular Biology
Zdroj:	Molecular and Cellular Biology. 2:701-707
ISSN:	1098-5549 0270-7306
DOI:	10.1128/mcb.2.6.701
Popis:	Nuclear RNA from Chinese hamster ovary cells was effectively separated into polyadenylic acid [poly(A)]-containing [poly (A)+] and non-poly(A)-containing [poly(A)−] fractions so that ∼90% of the poly(A) was present in the (A)+fraction. Only 25% of the 5′-terminal caps of the large nuclear molecules were present in the (A)+class, but about 70% of the specific mRNA sequences (assayed with cDNA clones) were in the (A)+class. It appears that many long capped heterogeneous nuclear RNA molecules are of a different sequence category from those molecules that are successfully processed into mRNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8b2b0931dfd04c078de056e44861ce69 https://doi.org/10.1128/mcb.2.6.701 Zobrazit plný text záznamu