Effects of L-Ornithine on Metabolic Processes of the Urea Cycle in Human Keratinocytes

Autor:	F. Höche, Johannes Wohlrab, T. Klapperstück
Rok vydání:	2004
Předmět:	Pharmacology Metabolic function Physiology L-Ornithine Dermatology General Medicine Biology Ornithine Arginase chemistry.chemical_compound chemistry Biochemistry Urea cycle Urea Ornithine metabolism Urea metabolism
Zdroj:	Skin Pharmacology and Physiology. 17:283-288
ISSN:	1660-5535 1660-5527
DOI:	10.1159/000081113
Popis:	The complex metabolic function of L-ornithine has led to a great interest in studying its pharmacotherapeutic potential. L-Ornithine is known to be crucial for the metabolism of keratinocytes, especially in the synthesis of urea, polyamines and precursors of collagen synthesis. In this study, we investigated in vitro the cytotoxicity of L-ornithine, and its influence on urea synthesis and arginase expression in primary human keratinocytes. L-Ornithine (≧1 mM) induced a decrease in the de novo urea synthesis of keratinocytes and an increase (≧10 mM) in the expression of the urea-generating enzyme arginase. Up to 20 mM, L-ornithine showed no cytotoxic potential, whereas higher concentrations induced apoptosis in keratinocytes in a concentration- and time-dependent manner.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8b1bca726fd3e07315778153409f33f8 https://doi.org/10.1159/000081113 Zobrazit plný text záznamu