A simple HPLC method for the simultaneous analysis of phosphatidylcholine and its partial hydrolysis products 1- and 2-acyl lysophosphatidylcholine
| Autor: | Ernst Wehtje, Dietlind Adlercreutz |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Aqueous solution
Chromatography Chemistry Elution General Chemical Engineering Organic Chemistry Oxalic acid High-performance liquid chromatography chemistry.chemical_compound Lysophosphatidylcholine Column chromatography Phosphatidylcholine lipids (amino acids peptides and proteins) Quantitative analysis (chemistry) |
| Zdroj: | Journal of the American Oil Chemists' Society. 78:1007-1011 |
| ISSN: | 0003-021X |
| DOI: | 10.1007/s11746-001-0379-8 |
| Popis: | A simple HPLC method for the simultaneous analysis of phosphatidylcholine (PC), 1-acyl lysophosphatidylcholine (1-acyl LPC), and 2-acyl lysophosphatidylcholine (2-acyl LPC) with refractive index detection is described. The separation of these three compounds was achieved on a Waters (Milford, MA) Spherisorb amino phase column using a mixture of ethanol and an aqueous oxalic acid solution as eluent. The optimal mixture of ethanol to oxalic acid solution was 92∶8 (vol/vol). PC and the two regioisomers of LPC eluted within 15 min. The calibration curves were linear in a concentration range from 0.05 to 2.5 mM. Natural PC or LPC eluted in a single peak, despite the diversity in the fatty acid composition. There was no rearrangement between 1-acyl LPC and 2-acyl LPC during analysis or storage in ethanol within 23 h. This method is thus especially suitable for studying reactions on PC and acyl migration in LPC. |
| Databáze: | OpenAIRE |
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