Internal validation of screening methodology for residues of epi-trenbolone, trenbolone, epi-nortestosterone and nortestosterone in bovine urine using immunoaffinity cartridges for sample clean-up

Autor:	Leslie C Dickson, Adrian C E Fesser, JoAnn Reid, Constance D Neiser, James D MacNeil
Rok vydání:	2002
Předmět:	Chromatography Chemistry Urine Repeatability Biochemistry Analytical Chemistry Clean-up Cartridge Target level Trenbolone Nandrolone medicine Environmental Chemistry Internal validation Spectroscopy medicine.drug
Zdroj:	Analytica Chimica Acta. 473:111-118
ISSN:	0003-2670
DOI:	10.1016/s0003-2670(02)00974-1
Popis:	This multi-analyte procedure uses commercially available immunoaffinity chromatography (IAC) clean-up cartridges for extraction and clean-up, followed by determination of residues of epi-trenbolone (ETB), trenbolone (TB), epi-nortestosterone (epi-nandrolone) (ENT) and nortestosterone (nandrolone) (NT) in bovine urine by liquid-chromatography with UV absorbance detection. This single-laboratory internal validation included assessment of recoveries, repeatability, linearity, detection capability and cross-reactivity (specificity) with a suite of antibiotics and other hormonal growth promotants (HGPs). The method was validated for screening at a target level of 2.0 ng ml−1.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8b13d2cbcc572b40e007cde20d2fb340 https://doi.org/10.1016/s0003-2670(02)00974-1 Zobrazit plný text záznamu Full Text from ScienceDirect