| Autor: | Takehide Murata, Nobuhiko Nomura, Kazunari K. Yokoyama, Christian Geltinger, Xiaoxian Zhao, Tadaatsu Nakahara, Kiyoshi Ohshima, Shotaro Kishikawa |
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| Rok vydání: | 2000 |
| Předmět: |
Ratón
Activator (genetics) Clinical Biochemistry Biomedical Engineering Bioengineering Cell Biology Cell cycle Biology Molecular biology carbohydrates (lipids) chemistry.chemical_compound chemistry Biochemistry Cell culture Apoptosis hemic and lymphatic diseases Phorbol Signal transduction neoplasms Protein kinase C Biotechnology |
| Zdroj: | Cytotechnology. 33:123-130 |
| ISSN: | 0920-9069 |
| DOI: | 10.1023/a:1008129616127 |
| Popis: | Mannosylerythritol lipid (MEL), an extracellularglycolipid from yeast, induces the differentiation ofHL-60 promyelocytic leukemia cells towardsgranulocytes. We show here that MEL is also a potentinhibitor of the proliferation of mouse melanoma B16cells. Flow-cytometric analysis of the cell cycle ofMEL-treated B16 cells revealed the accumulation ofcells in the sub-G0/G1 phase, which is a hallmark ofcells undergoing apoptosis. Treatment of B16 cellsfor 24 h with phorbol 12-myristate 13-acetate (PMA),an activator of protein kinase C (PKC), did notinterfere with the growth and survival of the cells,but it effectively counteracted the MEL-induced growtharrest and apoptosis. The activity of PKC was reducedin B16 cells treated with MEL at a concentration atwhich MEL induced apoptosis. However, incubation withPMA in addition to MEL reversed this reduction in theactivity of PKC. These results suggest thatconverging signaling pathways are triggeredindependently by MEL and PMA and that the signalsmight both be mediated by PKC. |
| Databáze: | OpenAIRE |
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