Catalytic properties of lipase adsorbed on nanocarbon-containing mesoporous silica in esterification and transesterification reactions
| Autor: | L. V. Perminova, G. A. Kovalenko, T. V. Chuenko, N. A. Rudina |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
food.ingredient
biology 010405 organic chemistry Ethyl acetate Transesterification Mesoporous silica equipment and supplies 010402 general chemistry Isoamyl alcohol 01 natural sciences Applied Microbiology and Biotechnology Biochemistry 0104 chemical sciences Catalysis chemistry.chemical_compound food Linseed oil chemistry Capric Acid biology.protein Organic chemistry Lipase |
| Zdroj: | Applied Biochemistry and Microbiology. 52:582-588 |
| ISSN: | 1608-3024 0003-6838 |
| DOI: | 10.1134/s0003683816060089 |
| Popis: | Nanocarbon-containing mesoporous silica covered with a varying amounts of nanostructured carbon of different morphologies were used as supports to immobilize Thermomyces lanuginosus lipase. The catalytic properties of the prepared biocatalysts were studied in both the transesterification of vegetable (linseed) oil in the presence of ethyl acetate and the esterification of the fatty acid (capric C10:0) in the presence of secondary (isopropyl or isoamyl) alcohols. The physico-chemical characteristics, such as the amount of adsorbed lipase, its specific activity, and the dependence of the activity and stability of the prepared biocatalysts on the support type were evaluated. The Michaelis-Menten kinetics was studied in the esterification of capric acid with isoamyl alcohol. The prepared biocatalysts were shown to retain up to 90% activity for >1000 h in the synthesis of isoamyl caprate. The half-time of the biocatalysts inactivation in the transesterification of linseed oil was found to be more than 700 h at 40°C. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|