Isothermal amplification and graphene based detection of circulating multiplex miRNAs

Autor:	Patraporn Luksirikul, Tararaj Dharakul, Deanpen Japrung, Kiatnida Treerattrakoon
Rok vydání:	2016
Předmět:	chemistry.chemical_compound chemistry Graphene law Blood circulation High-throughput screening microRNA Loop-mediated isothermal amplification Multiplex Non-coding RNA Molecular biology DNA law.invention
Zdroj:	2016 IEEE 16th International Conference on Nanotechnology (IEEE-NANO).
Popis:	This study developed simple multiplex miRNA amplification and detection platform using the combination of RCA approach and fluorescence quenching property of reduced graphene oxide. miRNA 29 and miRNA 144*, which are short noncoding RNA (22 nt) found highly expressed in the blood circulation of tuberculosis patients, are chosen for the development model. Our combination approaches could detect miRNA with the high sensitivity and has a potential for development of the high throughput screening and detection of circulating miRNAs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8abdb260049e9833c794900a59a8e103 https://doi.org/10.1109/nano.2016.7751320 Zobrazit plný text záznamu