Implementation of a semi-automated processing system for DNA profiling of forensic casework samples

Autor: Werner Pflug, Kai Vollack, Ralph Schwenzer, Barbara Haak
Rok vydání: 2008
Předmět:
Zdroj: Forensic Science International: Genetics Supplement Series. 1:83-85
ISSN: 1875-1768
DOI: 10.1016/j.fsigss.2007.11.006
Popis: The concept for a semi-automated processing system for DNA analysis of crime scene samples was developed at the Landeskriminalamt Baden-Wurttemberg (LKA BW) and comprises the extraction of genomic DNA from human cells by ChargeSwitch ® magnetic bead technology (CST), quantification of purified DNA by real-time PCR, amplification of short tandem repeats (STRs) by PCR and DNA fragment length analysis of STRs by capillary electrophoresis. Three liquid handling workstations from Tecan, a real-time PCR device and a 16-channel capillary electrophoresis (CE) system, both from Applied Biosystems (AB), are linked via laboratory data network. Transmission and management of sample and analysis data is enabled by a Laboratory Information and Management System (LIMS). Suitability for a wide range of stain types, early exclusion of DNA-free samples, barcode sample identification and prevention of cross-contaminations guarantee efficiency and high quality standards.
Databáze: OpenAIRE