| Autor: |
Qingjie Liu, Tianpeng Xie, Guilan Xue, Duan Fan, Xue Gou, Lingda Meng |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
AIM |
| DOI: |
10.1109/aim.2019.8868833 |
| Popis: |
The electromechanical properties of biomaterial are often essential to cell function during cell-biomaterial interaction. We describe the design and validation of an electromechanical testing and loading system for live cell study while co-cultured with biomaterial. The system is extended based on an uniaxial cell stretching device, and we use it to overcome a significant limitation of existing cell stretching devices, in which only strain are considered for cell stimulation. Combining proper mechanical design with high-precision electrical and force sensing, our system can simultaneously examine the mechanical and electrical stimulation on cell function caused by biomaterial. We validate our system by culturing mesenchymal stem cells on piezoelectric nanofiber membrane. The piezoelectric response of the nanofiber membrane is investigated under simulated in vivo stretch loading. Live cell imaging using an inverted microscope are implemented to clarify the manipulation principle of the device. Experiment results demonstrate that our system provides a useful tool for in vitro studies of electromechanical coupling effects of biomaterial on cell. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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