Callus Formation and Regeneration of Adventitious Embryos from Celery Microspores by Anther and Isolated Microspore Cultures
| Autor: | Sachiko Matsubara, Kenji Murakami, Norio Dohya |
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| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Engei Gakkai zasshi. 65:747-752 |
| ISSN: | 1880-358X 0013-7626 |
| DOI: | 10.2503/jjshs.65.747 |
| Popis: | Anthers and microspores of Apium graveolens L. (celery) were cultured in vitro to obtain haploid plants.Anther culture: Immature anthers of 'Cornell 619' and 'Cornell 19' at three stages of development were cultured on eight different media containing a half strength of Murashige and Skoog (1/2 MS) and B5 media supplemented with combinations of 2, 4-D, BA, NAA, and zeatin plus sucrose and Gelrite from May to June in 1994.Calli developed from 'Cornell 19' microspores at tetrad stage on 1/2 MS medium supplemented with 2, 4-D and B5 medium supplemented with 2, 4-D. In 'Cornell 619', calli developed from tetrad microspores on B5 medium supplemented with 2, 4-D, and from early uninucleous microspores on B5 medium supplemented with 2, 4-D. Adventitious embryos regenerated from calli transferred to MS medium with or without NAA and BA; plantlets grew after 40 to 60 days.Isolated microspore culture: Celery microspores at different developmental stages were cultured in liquid B5, NLN, and 1/2 MS media supplemented with 2, 4-D and BA, with or without glutamine and with serine at the density of 2.5 x 103 microspores/ml medium. In 'Cornell 619', many microspores of early uninucleate stage developed to colonies in the B5, NLN, and 1/2 MS media; the colonies in B5 and 1/2 MS media developed into heartshaped embryos; microspores, plated in modified 1/2 MS medium supplemented with 2, 4-D and BA, developed colonies and calli. |
| Databáze: | OpenAIRE |
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