Effect of optimization of the levels of dissolved oxygen in semen extender on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa
| Autor: | G. K. Das, A. R. Mustapha, S. K. Ghosh, R. Mustapha, J.K. Prasad, Med Ram Verma, Abhishek Kumar |
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| Rok vydání: | 2018 |
| Předmět: |
endocrine system
030219 obstetrics & reproductive medicine General Veterinary urogenital system Chemistry Extender 0402 animal and dairy science Semen 04 agricultural and veterinary sciences 040201 dairy & animal science Crossbreed Sperm Cryopreservation law.invention Andrology 03 medical and health sciences Semen quality Semen extender 0302 clinical medicine law Animal Science and Zoology Acrosome |
| Zdroj: | Indian Journal of Animal Research. |
| ISSN: | 0976-0555 0367-6722 |
| DOI: | 10.18805/ijar.b-3585 |
| Popis: | The objective of present study was to investigate the effects of two different levels of dissolved oxygen (DO) i.e. 4 and 8 ppm in the extenders on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa following cryopreservation. Only those ejaculates that had a mass activity of ³ 3+ and individual progressive motility of ³ 70% were utilized. Each of the ejaculates were divided and extended to have 80×106 spermatozoa/ mL of extender in three groups i.e. Group I (control), Group II (4 ppm) and Group III (8 ppm). Progressive motility (%) was higher (p Lass Than 0.05) in Group II and III as compared to Group I at both pre-freeze and post-thaw stage. Per cent live sperm and HOST reactive sperm were higher (p Lass Than 0.05) in Group II and in Group II and III as compared to Group I at pre-freeze and post-thaw stage, respectively. Sperm abnormality (%) was lower (p Lass Than 0.05) in Group II at post-thaw stage. Per cent acrosome intact sperm did not differ (p>0.05) among the groups at pre-freeze stage, but, were higher (p Lass Than 0.05) in Group II and III as compared to Group I at post-thaw stage. In conclusion, cryopreservation of semen having DO level of 4 ppm in semen extender yielded better results followed by 8 ppm in terms of semen quality parameters and functional membrane integrity of crossbred bull spermatozoa. |
| Databáze: | OpenAIRE |
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